Manual


DIMERBOW is a web-server application that provides information about possible dimers present in crystallographic structures of GPCR. The application constantly are updated. The reason why it uses all the newests structures stored in Protein Data Bank related with GPCR.
Last update: 2020-03-05

Functions

DIMEROW mainly uses the python plot interactive module Bokeh v.1.0.4 and the application NGL 2.0.0 to display the structures on html. These programs gives to the user some filtering options with an interactive plot and the possibility to take a look of the structures without installing anything and interact with them. Below, it is explained the functions that DIMERBOW have:

Bokeh - Interactive plot tools

Bokeh have a lot of tools that the user can use to interact with the plot. Tools more detailed explain clicking in this link Bokeh - Usage tools.
DIMERBOW have some of them: Note: Some of these tools are desactivate by default. Click on to activate them.

NGL

NGL is a viewer for molecular visualization that gives a lot of possibilities to display a structure. Controls are avaliable clicking in this link MANUAL - Mouse & Keyboard control.
In the case of DIMERBOW, NGL displays somes options:

Enable lipids/ligands

Also, for each structure you can download the structure in pdb format clicking in the button.

Modes

DIMERBOW have two modes:

PDB structure mode:

The PDB structure mode display the best possible dimers originated from the PDB structures. In this mode the user can filter using some parameters provides by the application (explained at the end of this paragrah) to select the structure or structures that will like visualize.

Filters
In the above image you can see at the left some filters that you can apply:

MD simulations mode:

The MD simulations mode display the possible structure dimers after simulations. In this mode the user can filter using some parameters provides by the application (explained at the end of this paragrah) to select the structure that you will visualize. Also, you can display the "crystallographic structure" to compare.

Filters
In the above image you can see at the left some filters that you can apply:

Examples

Example - 1

This example shows how to use the crystallographic mode and download the structure.

Example - 2

This example shows how to use the simulation mode and download the structure.